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( A ) The target tissues including PGCs are painted in orange at E0, E2.5, and E4.5, respectively. ( B-D ) Images of LEL staining <t>with</t> <t>SSEA-1</t> or DDX4 signal in E2.5 chicken samples. The percentages represents the proportion of SSEA-1 positive (+)/negative (-) or DDX4+/- cells within the entire LEL+ cell population. ( E ) Images of triple staining with LEL+, SSEA-1+, and DDX4+ cells in E2.5 chicken samples. ( F ) The Venn diagram showing the relative abundance of SSEA-1+ and DDX4+ cells in LEL+ population. ( G, H ) Images of LEL staining with SSEA-1 signal in E0 and E4.5 chicken samples. White and yellow arrowheads indicate the cells presumed to be PGCs and other than PGCs.
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( A ) The target tissues including PGCs are painted in orange at E0, E2.5, and E4.5, respectively. ( B-D ) Images of LEL staining <t>with</t> <t>SSEA-1</t> or DDX4 signal in E2.5 chicken samples. The percentages represents the proportion of SSEA-1 positive (+)/negative (-) or DDX4+/- cells within the entire LEL+ cell population. ( E ) Images of triple staining with LEL+, SSEA-1+, and DDX4+ cells in E2.5 chicken samples. ( F ) The Venn diagram showing the relative abundance of SSEA-1+ and DDX4+ cells in LEL+ population. ( G, H ) Images of LEL staining with SSEA-1 signal in E0 and E4.5 chicken samples. White and yellow arrowheads indicate the cells presumed to be PGCs and other than PGCs.
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( A ) The target tissues including PGCs are painted in orange at E0, E2.5, and E4.5, respectively. ( B-D ) Images of LEL staining <t>with</t> <t>SSEA-1</t> or DDX4 signal in E2.5 chicken samples. The percentages represents the proportion of SSEA-1 positive (+)/negative (-) or DDX4+/- cells within the entire LEL+ cell population. ( E ) Images of triple staining with LEL+, SSEA-1+, and DDX4+ cells in E2.5 chicken samples. ( F ) The Venn diagram showing the relative abundance of SSEA-1+ and DDX4+ cells in LEL+ population. ( G, H ) Images of LEL staining with SSEA-1 signal in E0 and E4.5 chicken samples. White and yellow arrowheads indicate the cells presumed to be PGCs and other than PGCs.
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( A ) The target tissues including PGCs are painted in orange at E0, E2.5, and E4.5, respectively. ( B-D ) Images of LEL staining <t>with</t> <t>SSEA-1</t> or DDX4 signal in E2.5 chicken samples. The percentages represents the proportion of SSEA-1 positive (+)/negative (-) or DDX4+/- cells within the entire LEL+ cell population. ( E ) Images of triple staining with LEL+, SSEA-1+, and DDX4+ cells in E2.5 chicken samples. ( F ) The Venn diagram showing the relative abundance of SSEA-1+ and DDX4+ cells in LEL+ population. ( G, H ) Images of LEL staining with SSEA-1 signal in E0 and E4.5 chicken samples. White and yellow arrowheads indicate the cells presumed to be PGCs and other than PGCs.
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Image Search Results


( A ) The target tissues including PGCs are painted in orange at E0, E2.5, and E4.5, respectively. ( B-D ) Images of LEL staining with SSEA-1 or DDX4 signal in E2.5 chicken samples. The percentages represents the proportion of SSEA-1 positive (+)/negative (-) or DDX4+/- cells within the entire LEL+ cell population. ( E ) Images of triple staining with LEL+, SSEA-1+, and DDX4+ cells in E2.5 chicken samples. ( F ) The Venn diagram showing the relative abundance of SSEA-1+ and DDX4+ cells in LEL+ population. ( G, H ) Images of LEL staining with SSEA-1 signal in E0 and E4.5 chicken samples. White and yellow arrowheads indicate the cells presumed to be PGCs and other than PGCs.

Journal: bioRxiv

Article Title: The universal labeling and sorting of avian primordial germ cell utilizing Lycopersicon Esculentum lectin

doi: 10.1101/2024.05.09.593277

Figure Lengend Snippet: ( A ) The target tissues including PGCs are painted in orange at E0, E2.5, and E4.5, respectively. ( B-D ) Images of LEL staining with SSEA-1 or DDX4 signal in E2.5 chicken samples. The percentages represents the proportion of SSEA-1 positive (+)/negative (-) or DDX4+/- cells within the entire LEL+ cell population. ( E ) Images of triple staining with LEL+, SSEA-1+, and DDX4+ cells in E2.5 chicken samples. ( F ) The Venn diagram showing the relative abundance of SSEA-1+ and DDX4+ cells in LEL+ population. ( G, H ) Images of LEL staining with SSEA-1 signal in E0 and E4.5 chicken samples. White and yellow arrowheads indicate the cells presumed to be PGCs and other than PGCs.

Article Snippet: Subsequently, the cells were incubated with a 1:500 dilution of anti-DEAD-box helicase 4 rat IgG antibody ( ) and a 1:100 dilution of anti-stage-specific embryonic antigen-1 (SSEA-1) mouse IgM antibody (sc-2170; Santa Cruz Biotechnology, Dallas, TX, USA) with 1% blocking reagent TNT for 1 hour on ice.

Techniques: Staining

( A ) The images of quail and emu blood PGCs stained by LEL. ( B ) Representative images of quail and emu PGCs, stained by LEL, DDX4, and SSEA-1. ( C ) Quail blood at HH14-15 was sorted and gate B (yellow square) was used for cell sorting. Histogram of FITC fluorescent and dot-plots of forward scattering (FSC; x-axis) versus back scattering (BSC; y-axis). Red arrowhead indicates the main subset of the distribution of the cells in gate B.

Journal: bioRxiv

Article Title: The universal labeling and sorting of avian primordial germ cell utilizing Lycopersicon Esculentum lectin

doi: 10.1101/2024.05.09.593277

Figure Lengend Snippet: ( A ) The images of quail and emu blood PGCs stained by LEL. ( B ) Representative images of quail and emu PGCs, stained by LEL, DDX4, and SSEA-1. ( C ) Quail blood at HH14-15 was sorted and gate B (yellow square) was used for cell sorting. Histogram of FITC fluorescent and dot-plots of forward scattering (FSC; x-axis) versus back scattering (BSC; y-axis). Red arrowhead indicates the main subset of the distribution of the cells in gate B.

Article Snippet: Subsequently, the cells were incubated with a 1:500 dilution of anti-DEAD-box helicase 4 rat IgG antibody ( ) and a 1:100 dilution of anti-stage-specific embryonic antigen-1 (SSEA-1) mouse IgM antibody (sc-2170; Santa Cruz Biotechnology, Dallas, TX, USA) with 1% blocking reagent TNT for 1 hour on ice.

Techniques: Staining, FACS